Composite

Part:BBa_K1780006:Design

Designed by: Snehal Kadam   Group: iGEM15_IISER_Pune   (2015-09-17)


LacI+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The oscillator module depends on the binding of lacI to its binding site to repress the promoter. It was necessary to find a LacI protein coding sequence with a degradation tag so it would not bring in any time lag in these oscillations.


Source

This part comes from previous biobricks

References